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Product

CD28 (human):Fc (human) (rec.)

Product Data

Synonyms : 

CD28

Properties

Host :

CHO cell

Sequence :

The extracellular domain of human CD28 (AAA60581.1) (Asn19-Pro152) is fused to the N-terminus of the Fc region of human IgG1.

Molecular Mass:

41 KDa (monomer)

Purity :

>98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

 

 

Activity:

Measured by its ability to inhibit 

IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells.

Shipping and Handling

Formulation :

Lyophilized from 0.2μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

CD28 and CTLA-4, together with their ligands, B7-1and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfidelinked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is downregulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is upregulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20 - 100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the upregulation of bcl-XL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.

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