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Product

B7-H4 (human):Fc (mouse) (rec.)

Product Data

Synonyms : 

V-set Domain-containing T Cell Activation Inhibitor 1; VTCN1; 

Immune Costimulatory Protein B7-H4; T Cell Costimulatory Molecule B7x 

Properties

Host :

CHO cell

Sequence :

The extracellular domain of human B7-H4(NP_078902.2)(Phe29-Ala258) is fused to the N-terminus of the Fc region of mouse IgG2a.

Molecular Mass:

55 KDa (monomer)

Purity :

>98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

 

 

Activity

Measured by the ablility to inhibit anti-CD3-induced proliferation of stimulated human T cells. 

Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days 

in 96 well place coated with 500 ng/ml antiCD3 and 10 ug/ml human B7H4/Fc. 

The presence of human B7H4/Fc at 10 ug/ml inhibited anti CD3 response by 30-50%.

Optimal dilutions should be determined by each laboratory for each application.

Shipping and Handling

Formulation :

Lyophilized from 0.2μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

B7H4 is a B7 family member that negatively regulates T cell immunity by inhibiting of T cell proliferation, cytokine production, and cell cycle progression. In vitro, B7H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production, and generation of alloreactive cytotoxic T-cells (CTLs). In vivo, blockade of endogenous B7-H4 by specific monoclonal antibody promotes T cell responses. B7-H4 ia an important negative regulator of innate immunity through growth inhibition of neutrophils. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance.

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