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CD275 [B7-H2] (mouse):Fc (mouse) (rec.) (non-lytic)

Product Data

Synonyms :  

B7-H2; ICOS Ligand; B7-like Protein Gl50; B7RP-1 


Host :

CHO cell

Sequence : 

The extracellular domain of mouse CD275 [B7-H2] (NP_056605.1)(Thr 48-Lys279) is fused to the 

N-terminus of the Fc region of a mutant mouse IgG2a

Molecular Mass

56 KDa (monomer)


Acts as a long lasting fusion protein which only binds to the receptor. 

Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins 

incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Purity :

≥98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.


Measured by its binding ability in a functional ELISA.

Immobilized human ICOS at 1 μg/ml (100μl/well) can bind biotinylated   B7-H2(mouse):Fc (mouse) (rec.) (non-lytic) with a linear range of 0.1 - 1.0 μg/ml.

Optimal dilutions should be determined by each laboratory for each application.

Shipping and Handling

Formulation :

Lyophilized from 0.2μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :


Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.


Mouse B7-H2, also called CD275, B7RP-1, B7h, LICOS and GL50, is a member of the growing B7 family of immune costimulatory proteins. CD275 has been identified as the ligand for ICOS, a member of the CD28 family of co-stimulatory receptors. Mouse CD275 is expressed constitutively on resting B cells and at low levels on monocytes. The CD275/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

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