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Product

CD275 [B7-H2] (human):Fc (human) (non-lytic)

Product Data

Synonyms : 

B7-H2; B7H2; B7RP-1; B7RP1; CD275; B7-like Protein Gl50; ICOSL

Properties

Host :

CHO cells

Sequence :

The extracellular domain of human CD275[B7-H2] (NP_056074.1) (Met1-Thr256) 

is fused to the N-terminus of the Fc region of a mutant human IgG1.

Molecular Mass:

52 KDa (monomer)

Non-lytic:

Acts as a long lasting fusion protein which only binds to the receptor. 

Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins 

incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Purity :

>98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

 

Activity:

Measured by its ability to stimulate human T cell proliferation 

in the presence of anti-CD3. 

Shipping and Handling

Formulation :

Lyophilized from 0.2μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

Human B7-H2,also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1(PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20-25% amino acid identity. Human and mouse B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

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