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Product

CD274 [B7-H1/PD-L1] (mouse):Fc (mouse) (rec.) (non-lytic)

Product Data

Synonyms : 

PD-L1; Programmed Cell Death 1 Ligand Protein 1; PDCD1 Ligand 1; B7-H1; 

Properties

Host : 

CHO cells  

Sequence :

The extracellular domain of mouse CD274 [B7-H1] (NP_068693.1) (Phe19-Arg237) 

is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.

 

Molecular Mass:

53 KDa

Non-lytic:

Acts as a long lasting fusion protein which only binds to the receptor. 

Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins 

incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

 

 

Purity :

≥98%by SDS-PAGE under reducing conditions. 

Endotoxin Content :

<60 EU/mg as determined by LAL test.

 

 

Activity:

Measured by its ability to inhibit 

anti-CD3-induced proliferation of stimulated mouse T cells.

Shipping and Handling

Formulation :

Lyophilized from 0.2μm-filtered solution in PBS.

Reconstitution :

Reconstitute at 100μg/ml in sterile PBS.

Use / Stability :

 

Stable for at least 1 year after receipt when stored 

at -20°C. Working aliquots are stable for up to 3 months 

when stored at -20°C.

                        Avoid freeze/thaw cycles.

Documents
Description

Programmed death ligand 1 (PD-L1, B7-H1 or CD274) is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. CD274 has been identified as one of two ligands for programmed death 1 (PD-1), a member of the CD28 family of immunoreceptors. CD274 is widely expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. CD274 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. CD274 expression is also induced in dendritic cells and keratinocytes after IFN-γ stimulation. CD274 expression is also upregulated in a variety of tumor cell lines. Interaction of CD274 with PD-1 results in inhibition of TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses.. The CD274 - PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

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